Journal: medRxiv

Article Title: Reduced risk of placental parasitaemia associated with complement fixation on Plasmodium falciparum by antibodies among pregnant women

doi: 10.1101/2021.06.02.21258254

Figure Lengend Snippet: (A) Complement C5-9 fixation on CS2 pRBCs was tested by flow cytometry following opsonization with either antibodies from a subset of 7 PNG donor samples (with moderate to high C1q/C3 fixing activity on CS2 pRBCs) or 6 Australian malaria non-exposed donors in the presence of NS. Complement fixation is presented as the average gMFI of each sample tested in duplicate and the red line represents the median gMFI for all samples tested in each category. (B) Correlation between complement C5-9 fixation and C1q/C3 fixation tested on a subset of 7 PNG donor samples (with moderate to high C1q/C3 fixing activity on CS2 pRBCs). (C) Complement-mediated pRBC killing/lysis was examined by culturing CS2 pRBCs opsonized with antibodies from PNG pregnant women (n=7) in the presence of normal serum, heat-inactivated serum or no-added serum and measuring parasitaemia by flow cytometry after two growth cycles. Data are presented as the average parasitaemia of each sample run in triplicate over 3 independent experiments. The dotted red line represents the median parasitaemia of CS2 pRBCs cultured in the absence of either PNG or Australian malaria non-exposed antibodies and NS or HIS. Comparisons were made using Mann-Whitney U-test.

Article Snippet: Pigmented trophozoite stage parasites at ∼10% parasitaemia and 0.5% haematocrit were incubated with 25% NS and 10% antibodies from PNG pregnant women or Australian malaria non-exposed donors for 1 hour at 37°C. pRBCs were then washed in 0.5% BSA in PBS and C1q, C3 and C5-9 detected by staining with polyclonal rabbit anti-human C1q, goat anti-human C3 or monoclonal mouse anti-human C5-9 antibodies followed by respective polyclonal Alexa-488 conjugated goat anti-rabbit, rabbit anti-goat or goat anti-mouse antibodies. pRBCs were detected by staining with ethidium bromide (Bio-Rad) at 1/1000 dilution.

Techniques: Flow Cytometry, Activity Assay, Lysis, Cell Culture, MANN-WHITNEY

Journal:

Article Title: ?? T cells in Beh?et's disease (BD) and recurrent aphthous stomatitis (RAS)

doi: 10.1046/j.1365-2249.1999.01069.x

Figure Lengend Snippet: The expression of αβ T cell receptor (TCR) on peripheral blood lymphocytes (PBL) and expression of CD4 or CD8 on αβ T cells (a) and the expression of γδ TCR or CD56 on PBL and expression of CD4 or CD8 on γδ T cells (b), analysed by double immunofluorescent staining and flow cytometry. Results are expressed as percentage positive cells, shown as mean ± s.e.m. for 20 BD patients (▪), 11 RAS patients (hatched bars) and 20 healthy controls (□). Statistically significant differences between BD patients and controls, BD patients and RAS patients, and RAS patients and controls are indicated.

Article Snippet: MoAbs against human CD25 (clone M-A251) and FITC-labelled MoAbs against human CD56 (clone C5.9) were purchased from Serotec (Oxford, UK).

Techniques: Expressing, Staining, Flow Cytometry